Oxidative Stress in Semen

There is growing evidence to support a link between oxidative stress and male infertility.

Oxidative Stress is the underlying cause of sperm DNA damage. It impairs semen parameters and fertilisation, adversely affects embryo development, and is associated with reduced pregnancy rates. It may also increase the risk of miscarriage. High levels of ROS may be reduced by considering varicocele repair, treatment of underlying infections or inflammation, changes in lifestyle or with antioxidant supplements.

TDL provides a comprehensive assessment of oxidative stress by combined measurement of Reactive Oxygen Species and Redox Potential. Please request as oxidative stress test (code ROS).

The test includes combined testing for:

Chemiluminescence Assay for Reactive Oxygen Species

Reactive Oxidative stress may be measured by a simple chemiluminescence test which measures the level of reactive oxygen species in semen.

MIOXSYS Electrochemical Assay for Redox Potential

Oxidative stress may be determined by an electrochemical assay which measures the redox potential in semen. This test measures the overall difference between total oxidants and antioxidants in the system.

 

References

Homa ST, Vessey W, Perez-Miranda A, Riyait T, Agarwal A (2015). Reactive oxygen species (ROS) in human semen: determination of a reference range. J Assist Reprod Genet 32(5):757-64. Full text

Vessey W, Perez-Miranda A, Macfarquhar R, Agarwal A, Homa S. (2014). Reactive oxygen species (ROS) in human semen: validation and qualification of a chemiluminescence assay. Fertil Steril. 102:1576-1583. Abstract